Etiologic Risk Group (see link below): Risk Group 2
*/Disease Information (sort of like the Intro to your Mini Research Write up):
Subspecies of the African trypanosome, Trypanosoma brucei, which cause human African trypanosomiasis, are transmitted by the tsetse fly, with transmission-essential lifecycle stages occurring in both the insect vector and human host. During infection of the human host, the parasite is limited to using glycolysis of host sugar for ATP production. This dependence on glucose breakdown presents a series of targets for potential therapeutic development, many of which have been explored and validated as therapeutic targets experimentally. These include enzymes directly involved in glucose metabolism (e.g., the trypanosome hexokinases), as well as cellular components required for development and maintenance of the essential subcellular compartments that house the major part of the pathway, the glycosomes. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195984/
Essentiality: During infection of the human host, the parasite is limited to using glycolysis of host sugar for ATP production. This parasite's dependence on glucose breakdown presents a series of targets for potential therapeutic development, many of which have been explored and validated as therapeutic targets experimentally. These include enzymes directly involved in glucose metabolism (e.g., the trypanosome hexokinases and phosphoglycerate kinase), as well as cellular components required for development and maintenance of the essential subcellular compartments that house the major part of the pathway, the glycosomes. Link: https://www.hindawi.com/journals/mbi/2011/123702/
Phosphoglycerate kinase, glycosomal (PGKA)
*Target (protein/gene name):Phosphoglycerate kinase, glycosomal (PGKA)
*NCBI Gene # or RefSeq#: N/A
*EC#: 2.7.2.3
*Protein ID (NP or XP #) or Wolbachia#: 1QPG
*Organism (including strain): Crithidia fasciculata
Etiologic Risk Group (see link below): Risk Group 2
*/Disease Information (sort of like the Intro to your Mini Research Write up):
Subspecies of the African trypanosome, Trypanosoma brucei, which cause human African trypanosomiasis, are transmitted by the tsetse fly, with transmission-essential lifecycle stages occurring in both the insect vector and human host. During infection of the human host, the parasite is limited to using glycolysis of host sugar for ATP production. This dependence on glucose breakdown presents a series of targets for potential therapeutic development, many of which have been explored and validated as therapeutic targets experimentally. These include enzymes directly involved in glucose metabolism (e.g., the trypanosome hexokinases), as well as cellular components required for development and maintenance of the essential subcellular compartments that house the major part of the pathway, the glycosomes.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3195984/
Essentiality: During infection of the human host, the parasite is limited to using glycolysis of host sugar for ATP production. This parasite's dependence on glucose breakdown presents a series of targets for potential therapeutic development, many of which have been explored and validated as therapeutic targets experimentally. These include enzymes directly involved in glucose metabolism (e.g., the trypanosome hexokinases and phosphoglycerate kinase), as well as cellular components required for development and maintenance of the essential subcellular compartments that house the major part of the pathway, the glycosomes.
Link:
https://www.hindawi.com/journals/mbi/2011/123702/
Materials and Reagents: N/A
Link to BRENDA EC# page:
https://www.brenda-enzymes.org/enzyme.php?ecno=2.7.2.3
-- Show screenshot of BRENDA enzyme mechanism schematic:
Structure (PDB or Homology model)
-- PDB # or closest PDB entry if using homology model: 1QPG
-- For Homology Model option:
---- Show pairwise alignment of your BLASTP search in NCBI against the PDB
---- Query Coverage: N/A
---- Max % Identities: N/A
---- % Positives: N/A
---- Chain used for homology:
Druggable Target: N/A
Assayable Enzyme Target: N/A
Link to Brenda: https://www.brenda-enzymes.org/enzyme.php?ecno=1.11.1.21&Suchword=&reference=&UniProtAcc=&organism%5B%5D=Mycobacterium+tuberculosis&show_tm=0
Structure:
Current Inhibitors: N/A
Expression Information (has it been expressed in bacterial cells): Yes
Purification Method: N/A
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
Length of protein in amino acids: 415
Molecular Weight: 45356.61 kDa
Molar Extinction coefficient of your protein at 280 nm wavelength: 1.67 M-1*cm-1
*CDS Gene Sequence (paste as text only):
<span style="background-color: #ffffff; font-family: monospace,serif;"> [[#sequence_L25121.1]] 1 <span class="ff_line">cacaacgact catctaataa cattctctcc atctcttccc ttcactcacg tgtgcgccat</span> 61 <span class="ff_line">ccgacacacc cgtctattcc ggtgcctgtt tctctctaca gaacctcctt tttcttgttc</span> 121 <span class="ff_line">agctggctgc ctcaggcact gcgctcgcac cccctccgcc gagccatgtc tctcgtgctg</span> 181 <span class="ff_line">aagaagagca tcgatgacgc caccgtcagg gacaagaagg tgctcatccg cgtggacttc</span> 241 <span class="ff_line">aacgtccccg tgaagaacgg taagatcacg aatgacttcc gcattcgctc cgcccttccg</span> 301 <span class="ff_line">acgatccaaa aggtgctgaa ggagggcggc tcctgcatcc tgatgagcca ccttggtcgg</span> 361 <span class="ff_line">ccgaagggtg cgagaatgag tgacccaagt ccggagaagg gtgtgcgcgg gtacgaggag</span> 421 <span class="ff_line">gccgccacgc tgcgcccggt tgctgcacgc atcagcgagc tgctggggca gaaggtggag</span> 481 <span class="ff_line">tttgcaccgg actgcctgga cgctgcgtcg tacgcgtcga agctgaagaa cgcggacgtg</span> 541 <span class="ff_line">ctgttgctgg agaacgtgcg tttctacgcg gaggagggca gcaagaagga ggaggagcgc</span> 601 <span class="ff_line">gacgcgatgg cgaaggtact agcgtcgtac ggcgacgtgt atgtcagtga cgcgtttggc</span> 661 <span class="ff_line">actgcgcacc gcgacagcgc gacgatgacg ggcatcccca aggtgctggg tgcgggctac</span> 721 <span class="ff_line">gccgggtacc tgatggagaa ggagatcaac tacttctcgc gagtgctgaa caacccgccg</span> 781 <span class="ff_line">cggccgctgg ttgcgattgt cggcggtgcg aaggtcagcg acaagatcca gctgcttgac</span> 841 <span class="ff_line">aacatgctgg gccgcatcaa ctaccttgtg attggtggcg cgatggcgta cacgttccag</span> 901 <span class="ff_line">aaggcgcagg gccgcaagat cggcatctcg atgtgcgagg aggataagct ggaccttgcc</span> 961 <span class="ff_line">aagtcgctgc tgaagaaggc gcaagagcgg aatgtgcagg tgtttctgcc agttgaccac</span> 1021 <span class="ff_line">gtgtgcaaca aggaatttaa ggccgcggac tcgccgctgg tgacggagag cgtggacgtc</span> 1081 <span class="ff_line">ccggacggct acatggcgct ggacattggc ccaaggacga tccacatgta cgaggaagtg</span> 1141 <span class="ff_line">attggccgct gcaagagcgc gatctggaac ggcccgatgg gcgtgttcga gatgccgtgc</span> 1201 <span class="ff_line">tactcgaagg gcacatttgc cgttgcgaag gcgatgggga ctggaacgca gaagaacggg</span> 1261 <span class="ff_line">ctgatgagca tcatcggcgg tggtgacagc gcgagcgctg cggagctgag cggcgaggcg</span> 1321 <span class="ff_line">aagaacatgt cgcatgtgtc caccggcggc ggtgcgtccc tggagctgct ggagggcaag</span> 1381 <span class="ff_line">acgctgcctg gcgtcgccat tctgacggac aaggacgtga aggagcgggg agcttcgtgc</span> 1441 <span class="ff_line">aagttcgctt tcggggtcgg gagcccgagc agggaggctt gtccgctccg ttgtgggcat</span> 1501 <span class="ff_line">atctttggcg gtgcctccat cgtaagagag atagttaaga tcgtcgtcgc gctgctgatc</span> 1561 <span class="ff_line">ggcattttta ttggtcgccg catgagcacc aagctgatcc ggtaaggtgt acgctgcgtc</span> 1621 <span class="ff_line">tttttctctc cgtgcggaat ttcatctgag atgagcagca gcggcgccct tccggagaca</span> 1681 <span class="ff_line">gcgaacgagt atcggcggcc acccaagcta cttgcgcctg gcactggcgc aatgattgtt</span> 1741 <span class="ff_line">gttgctggca aaggatgcag gtgagtgggt gatggtggtg gagggcctgc ctgtctgtcg</span> 1801 <span class="ff_line">ctctgtgtgt gtgtgtgtgt gtgcctcacg tactcagcac tcttctttct tcttcgcttt</span> 1861 <span class="ff_line">ctagctcctc cttgtcgccc tcttgaagtc tgttgatgat gtacaagttt gtgtgttgtt</span> 1921 <span class="ff_line">ggtcaccgca gcgtgagccg ttgtgcgctc ttcgtctacc tccgttctct ccgtctctac</span> 1981 <span class="ff_line">aattttttct gtgtgtgtgt gtgtgaggaa gagagtgccg atgtgctgcg ccgtctcgac</span> 2041 <span class="ff_line">ggaaagcgca gcaggtggca aaaacatgcg tgaagagctt gagtggcggc agctgcacag</span> 2101 <span class="ff_line">aagcacttca gtcctcgagt gcgtgggcgt gtgctagttc acgggtattg gtgtgcgcga</span> 2161 <span class="ff_line">ctcttttcct gcctgccgct gccgtttttt ttttttcaac ttcgaacgtc ctgacttcgc</span> 2221 <span class="ff_line">cgctctagtc gtggcgctct cgaaggatcc</span> </span>*GC% Content for gene: 69.7%
*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):
*GC% Content for gene (codon optimized): 69.7%